In the present study, nickel sulfide (NiS) decorated reduced graphene oxide was synthesized by a facile one-step hydrothermal approach. Characterization of the as-made nanohybrid using a Field emission scanning electron microscope (FE-SEM) and powder X-ray diffraction (XRD) clearly demonstrate the successful attachment of NiS onto the rGO nanosheets. Further, the prepared NiS–rGO nanohybrid has been examined for the electrochemical nonenzymatic detection of glucose using cyclic voltammetry, linear sweep voltammetry and amperometry. The electrochemical studies demonstrated that the NiS–rGO nanohybrid modified electrode detects glucose linearly over a concentration range of 5.0 × 10−5 to 1.7 × 10−3 M with a detection limit of 1.0 × 10−5 M. The obtained detection limit for the NiS–rGO nanohybrid is very much comparable to the recent literature values. Further, the NiS–rGO nanohybrid modified electrode showed an excellent anti-interference ability against electroactive species and showed good reproducibility and stability.